Jan R. Assa, Simon B. Widjanarko, Joni Kusnadi, Siegfried Berhimpon
Antioxidant methanol extract potential of flesh, seed and mace of nutmeg (Myristica fragrans Houtt) were evaluated with methods of 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric-reducing antioxidant power (FRAP), ferrous ion chelating activity and antioxidant activity assay in a linoleic acid system with ferrothiocyanate reagent (FTC). Total phenol and phytochemical of the extract were also evaluated. Seed extract has the most powerful scavenging ability on free radicals based on test of DPPH and FRAP, i.e. 154,55 (IC50=μg/ml) and 82,33 (mg GAE/g extract) respectively. DPPH and FRAP value of mace extract is 201,97 and 56,31 respectively, while in flesh are 1372,91 and 13,45 respectively. Total phenol of mace, seed and flesh extract are 4.630,62, 2.434,90 and 388,36 mg GAE/g D.W. Total phenol correlated to DPPH (r=0,87) and FRAP (0,63). Flesh, seed and mace extract well inhibit the linoleic peroxidation. Linoleic peroxidation on up to 8 days incubation - expressed as absorbance in control 1.4450 - in flesh is 0.0337; seed 0.0330; and mace 0. 0310. Ferrous ion chelating activity orders are: flesh extracts gt; seed extracts gt; mace extracts. Tannin, flavonoid and terpenoid were also found in seed and mace extract, whereas flesh extract contain flavonoid and terpenoid.
University of Brawijaya, Indonesia; Departement of Agricultural Technology, Sam Ratulangi University, Indonesia; Departement of Agricultural Technology, Brawijaya University, Indonesia; Departement of Fishery Product Technology, Sam Ratulangi University, Indonesia
