Niken Candra Bestari, Suharjono
Fish processing industry at Muncar, Banyuwangi, produced wastewater containing fish oil and could be degraded into fatty acids and glycerol by lipase-producing bacteria. Aims of this research were to determinethe activity and identify bacteria that have the highest lipase activity based on 16S rDNA sequences derived from fish mill wastewater at Muncar, Banyuwangi. Bacteria were isolated using simple mineral media, obtained isolates were assayed qualitatively based on the clear zone diameter by using 2 % olive oil as substrate. Clear zone diameter on the 7th day of incubation were analyzed by One-Way ANOVA(p=0,05). The lipase activity of four isolates with the highest diameter, 1me (4.08 ± 0.21 mm), 3ma(3.94 ± 0.03 mm) 3mb (3.99 ± 3.15 mm), and 3mc (4.02 ± 0.15 mm) were assayed simultaneously with the growth curve of bacteria. The highest lipase activity of 4.05 U/ml was produced by 3mc at the 34th hours incubation. Bacterial 3mc isolate were identified based on 16S rDNA sequence with 27F and 1492F primers. The identification results showed that 3mc has the 100 % similarity 16S rDNAsequence with Staphylococcus aureus B3. © 2015, Sphinx Knowledge House. All rights reserved.
Department of Biology, Brawijaya University, Indonesia
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