H.Y. Lukman, W. Busono, S. Wahyuningsih, S. Suyadi
This study aims to determine the effect of adding α - tocopherol in Tris Aminomethane - egg yolk extender towards sperm motility and viability of Bali bull during storage at temperature of 5°C. Semen of Bali bull was obtained from breed in the Center of Regional Artificial Insemination of Mataram. The semen was diluted with Tris egg yolk then divided into four doses treatment of α – tocopherol: 0.0; 0.2; 0.4 and 0.6 g/100 ml of diluent, respectively. Motility and viability of sperm stained by eosin-negrosin and observed with luminescent microscope. Semen which has been stored at temperature of 5°C was observed daily for 8 days. The result show that the doses of α - tocopherol in Tris aminomethane - egg yolk diluent is significantly (P < 0.05) give a better percentage on sperm motility and viability of Bali Bull. The best result was showed by doses of 0.4 g α – tocopherol. It preserved the sperm motility for 47 ± 5.9 and viability for 73.9 ± 3.81 until 8 days of storage at temperature of 5°C. The percentage of sperm motility and viability of Bali Bull which had been stored at temperature 5°C for 8 days is remain in a good quality to be used for Artificial Insemination (AI) of liquid semen. © 2014 Sphinx Knowledge House. All rights reserved.
Department of Animal Production, University of Mataram, Pendidikan Street No. 62, Mataram, 83114, West Nusa Tenggara, Indonesia; Department of Animal Production, University of Brawijaya, Veteran Street, Malang, 65145, East Java, Indonesia
