Erwien Isparnadi, Moh Hidayat, Aulanni’Am Aulanni’Am, Nur Permatasari
Atrophic non-union fracture is a further complication of a fracture in the form of non-bridging callus formation between the two fracture fragments accompanied by atrophy of the cells and tissue at the ends of the fragments and necrosis, thereby resulting in avascular defect. This complication is very difficult to manage and requires a operation of fixation and bone graft. Patients will experience a reduced quality of life due to the function of a limb is decreased and even reduced completely. The discovery of mesenchymal stem cells gives a promising for optimal treatment. Mesenchymal stem cells (MSCs) are adult stem cells produced by human bone marrow that will differentiate into osteoblasts. A vascular defect requires biomaterial (scaffold) and Platelet Rich Plasma (PRP). Scaffold is used to connect both ends of the defect, while the PRP is used to stimulate angiogenesis thereby resulting in neovascularization. This research used hidroxyapatite (HA) scaffold, which is used as an allograft, Which was from the scallops shells (Bivalves anodonta-BA). It produced biomaterial Chitosan, and it was expected to be new reported. This study used the post test only control group design with animals models of male New Zealand White Rabbit. The animals were divided into 3 groups (G1, G2, G3). Animal models were made by cutting the ulna bone and then cauterization on soft tissue and periosteum was made around the tip of the fracture. After 8 weeks, the defect was implanted with MSCs + PRP (G1), MSCs + PRP + Hidroxyapatite (G2), MSCs + PRP + BA (G3). Assay was conducted with immuno histochemistry for osteocalcin and collagen type I expression. The result of immuno histochemistry assay for expression of osteocalcin, collagen type 1 and histopathological assay showed that scaffold played very important role in the healing of atrophic non-union fracture that confirmed based on osteocalcin and collagen type I. In Conclusion, bone regeneration process was better in the groups implanted with MSCs + PRP + BA compared with the hydroxyapatite (HA) scaffold or without scaffold. © 2015, International Journal of ChemTech Research. All right reserved.
Brawijaya University, Indonesia; Department of Orthopedics and Traumatology, Haji General Hospital, Surabaya, Indonesia; Department of Orthopedics and Traumatology, Saiful Anwar General Hospital, Malang, Indonesia; Laboratory of Biochemistry, Brawijaya University, Indonesia; Department of Pharmacology, Brawijaya University, Indonesia
