Celcius Waranmaselembun, Eddy Suprayitno, Aulanni Am, Andhardoko
Inflammatory Bowel Disease (IBD) is an inflammatory disease occurring in gastrointestinal tract, particularly colon, that can result from the side effect of antiinflammatory non-steroid (AINS) drug usage, such as Indomethacine. “Mas ngur”oysters (Atactodea striata) have long been known by people of Kei-Southeast Mallucasas traditional drug, but their use as antiinflammation has never been studied yet. This study was intended to measure the active compound ability of the oyster to reduce the protease activity and occludin increment in rat’s (Rattus norvegicus) ileum with indomethacine-induced IBD. Test animals were 8-12 weeks old-male rats of 150-200 grams. They were separated into 3 groups, healthy, sick (induced with 15 mg/kg BW indomethacine), therapy goups (15 mg/kg BW indomethacine oral induction then treated with mas ngur oyster powder of100, 400, 700 mg/kg BW). Indomethacine induction of 15 mg/kg BWand therapy of mas ngur oyster powder extract were administered orally. The protease activity was measured using a spectophotometer, while the occludin expression was measured using immunohistochemistry. Results showed that the extract therapy gave significantly different effect (P<0.05) among treatments with effective dose of 400 mg/kg BW that could reduce the protease activity to 52.03%, andincrease the occludin expression to 989.706%. © 2015, Sphinx Knowledge House. All right reserved.
Brawijaya University, East Java, Indonesia; State Fisheries Polytechnique Tual, Southeast Mallucas, Indonesia
