Adell M. Ahmed Abubakeer, Sri Winarsih, Hidayat Sujuti
Garcinia mangostana L pericarp extract is known has active substances called Xanthones which has strong antioxidant effects. This substance also has anti-inflammatory and immunomodulation effects. However, the intercellular infection of Salmonella stimulates macrophage to produce interleukin IL12 for enhancement of IFN-γ secretion. lFN γ, which plays role for activates macrophage, acts as positive feedback in stimulating macrophages to kill S. Typhimurium. Macrophages play role in the phagolysosome fusion process are produced. This study aims to determine the effect of Crude Ethanolic Mangosteen Extract (CEME) on IFN-γ and IL-12 secretion in mice infected with S. Typhimurium. This study used 25 mice Balb/c. Mice divided into 5 groups consist of 5 mice in each group, including positive control (mice were infected with S. Typhimurium), T0 (control with 40 mg/ml of crude extract mangosteen pericarp without S. Typhimurium), T1, T2 and T3 (treatment with 20 mg/ml, 40 mg/ml and 60 mg/ml of crude extract mangosteen pericarp). Then, the mice were necropsied and dissected to take the blood directly from the heart. Levels of IFN-γ and IL-12 were analyzed using ELISA. Data were analyzed by One-Way ANOVA followed by Post-Hoc test (LSD) to assess the comparison between groups. Results showed that CEME increase serum IFN-γ and IL12 without infection. CEME increase serum IL12 in T1, T2and T3 with a p-value 0,000. Other results showed decreases serum IFN- γ in T2 and T3 with a pvalue 0,000. CEME improve immune response through the increases serum IL-12 and decreases serum IFN-γ in mice infected with S. Typhimurium. © 2015, Sphinx Knowledge House. All right reserved.
Laboratory of Microbiology, Brawijaya University, JI Veteran Malang, East Java, 65145, Indonesia; Laboratory of Pharmacology, Brawijaya University, JI Veteran Malang, East Java, 65145, Indonesia
