Dharma P.T.R. Maluegha, M. Aris Widodo, Bambang Pardjianto, Edi Widjajanto
This study aimed to investigate the effects of electrical injury on vascular damage and on matrix metalloproteinase (MMPs) levels. Thirty male Wistar albino rats were divided into five groups (. n=6), including a control group (untreated group), a 600mv electrical burn injury group, a 900mv electrical burn injury group, a 1200mv electrical burn injury group, and a 1500mv electrical burn injury group. Endothelial progenitor cells (EPCs) and circulating endothelial cells (CECs) were analyzed using flow cytometry. The levels of MMP-9, MMP-3, nitric oxide (NO), vascular endothelial growth factor (VEGF), and vascular cell adhesion molecule (VCAM) were assayed using an enzyme linked immunosorbent assay. Levels of EPCs were significantly lower in all electrical burn injury groups compared with the control group (. p<0.05). The levels of NO and VEGF were significantly increased in all electrical burn injury groups compared with the control group (. p<0.05). The levels of MMP-9 were significantly higher in the 1200mv electrical burn injury group compared with the control group or the 900mv electrical burn injury group (. p<0.05). There were no significant differences inMMP-3, VCAM, and CECs levels between groups (. p>0.05). This electrical burn injury model shows a significant decrease in endothelial progenitor cells and an increase in VEGF, NO, and MMP-9 as the compensating mechanism. © 2015 .
Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia; Pharmacology Laboratory, Faculty of Medicine, University of Brawijaya, Malang, East Java, Indonesia; Department of Plastic Surgery, Saiful Anwar General Hospital, Faculty of Medicine, University of Brawijaya, Malang, East Java, Indonesia; Clinical Pathology Laboratory, Saiful Anwar General Hospital, Faculty of Medicine, University of Brawijaya, Malang, East Java, Indonesia
