B. Tong, G.Q. Gao, Y. Muramatsu, T. Ohta, H. Kose, G.P. Li, F. Fatchiyah, T. Yamada
The septin 7 (. CDC10) gene, involved in cellular proliferation, has been previously shown to be expressed at different levels in the longissimus muscle (LM) between low-marbled and high-marbled steer groups by differential-display PCR. It is located within the genomic region of a quantitative trait locus for marbling, and thus was considered as a positional functional candidate gene for marbling. In this study, we showed that the CDC10 expression levels in the LM were positively correlated with marbling in Japanese Black (JB) steers (. P<. 0.0001). Further, an association analysis indicated that a SNP in the promoter region of the CDC10 gene was associated with marbling using 99 JB sires (. P=. 0.03) and 542 JB paternal half-sib progeny steers from a sire homozygous for G allele at the SNP (. P<. 0.0001). These findings suggest possible effects of the expression levels in the LM and the SNP of the CDC10 gene on marbling in JB cattle. © 2015 Elsevier Ltd.
The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, China; Department of Agrobiology, Faculty of Agriculture, Niigata University, Nishi-ku, Niigata, Japan; Department of Nutritional Sciences for Well-being, Faculty of Health Sciences for Welfare, Kansai University of Welfare Sciences, Kashiwara, Osaka, Japan; Central Pharmaceutical Research Institute, Japan Tobacco, Inc., Takatsuki, Osaka, Japan; Department of Life Science, Division of Natural Sciences, International Christian University, Mitaka, Tokyo, Japan; Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, East Java, Indonesia
