Abdul Manab, Manik Eirry Sawitri, Khotibul Umam Al Awwaly, Aris Sri Widati, Yuny Susanti Haniyah
The aim of this research were to find out the effect of sodium cyanoborohydride addition on the release of modified lysozyme from composite edible film based on whey protein and porang flour. The research was the addition of sodium cyanoborohydride on modified lysozyme aquaeous (control; 0.005%; 0.007% and 0.009%) using randomyzed block design, the variables were, the release of modified lysozyme, antibacterial of modified lysozyme on Latobacillus bulgaricus and Escherichia coli, water vapor permeability, protein solublity and microstructure of composite edible film. The results were the treatment didn’t gave significantly effect (P>0.05) on release of modified lysozyme, antibacterial of modified lysozyme, water vapor permeability, protein solublity and microstructure of composite edible film. Increasing sodium cyanoborohydride produced decreasing the release of modified lysozyme from composite edible film, however, the treatment produce stable antibacterial activity, water vapour permeability and protein solubility of composite edible film containing modified lysozyme and sodium cyanoborohydride. Formation of more dense structure with increased sodium cyanoborohydride content. It concluded that Sodium cyanoborohydride contributed to controlled relesase rates of modified lysozyme from composite edible film by reducing film porosity. The film showed antimicrobial activity against Latobacillus bulgaricus and Escherichia coli. © 2016, Sphinx Knowledge House. All rights reserved.
Animal Husbandry Faculty Brawijaya University, Jalan Veteran Malang, 65145, East Java, Indonesia; Agriculture and Animal Husbandry Polytechnic, MAPENA Tuban East Java, Indonesia