C. Padaga Masdiana, Aulanni’am Aulanni’am, Hidayat Sujuti, Widodo
There is an increasing amount of evidence that oxidative stress related to hypertension and damages the function of aorta and kidney. It is a well established fact that food protein, especially milk casein generates bioactive peptides with antihypertensive and antioxidative activities. The present study sought to investigate blood pressure lowering effect and antioxidative activity of the casein derived from goat milk yogurt in Deoxycorticosterone acetate (DOCA)-salt induced hypertensive rats (Rattus norvegicus). Casein were separated from the goat milk yogurtby centrifugation.The rats were orally administrated with casein in daily dose of 300 and 600 mg/kg body weight for 4 weeks. Systole blood pressure (SBP), renal and aorta total malondialdehyde (MDA) and histopathology of kidney and aorta abdominalwere examined. Mean of SBP were lowered in the hypertensive rats following casein therapy from their respective values observed at the time of administration. Renal and aorta injury was observed in DOCA-salt hypertensive group rats compared to normotensive group rats, as renal and aorta MDA significantly increased (P <0.05). In contrast, treatment of DOCA-salt hypertensive rats with different dose of casein significantly reduced the total renal and aorta MDA, as well as repair kidney damage, suppressed smooth muscle cell proliferation and lessen aorta wall thickening compared to controls. This is the first report that demonstrated blood pressure lowering and antioxidative effectsof casein derived from goat milk yogurt in DOCA-salt model of hypertension. © 2015, Sphinx Knowledge House. All right reserved.
Brawijaya University, Malang, 65145, Indonesia; Biochemistry laboratory, Brawijaya University, Malang, 65145, Indonesia; Biochemistry Laboratory, Brawijaya University, Malang, 65145, Indonesia; Department of Biology, Brawijaya University, Malang, 65145, Indonesia
