Pei-Shiuan Su, Robert J. Doerksen, Sin-Hong Chen, Wang-Chou Sung, Chia-Chi Juan, Reynetha D.S. Rawendra, Chiy-Rong Chen, Jia-Wei Li, Aisha, Tzou-Chi Huang, Ming-Huei Liao, Chi-I Chang, Jue-Liang Hsu
In this study, we screened 10 resveratrol derivatives isolated from Ampelopsis brevipedunculata var. hancei (Planch.) Rehder (ABH) for angiotensin I converting enzyme (ACE) inhibitory (ACEI) activity. Among these compounds, (+)-hopeaphenol and (+)-vitisin A showed the lowest IC50 values (~1.5μM) toward ACE. In addition, the compounds' abundances and distributions in ABH were profiled using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Interestingly, trimers and tetramers of resveratrol were mainly obtained from the bark of ABH when 90% ethanol was used for extraction. This result implies that the antihypertension effect of ABH extract may be mainly contributed by (+)-hopeaphenol (F1) and (+)-vitisin A (F2) in the ABH bark due to their remarkable ACE inhibitions. Moreover, the sizes and structures of these compounds were further correlated to their affinities toward ACE using molecular docking calculations. The results showed that resveratrol tetramers interact with ACE more favorably than other smaller oligomers. © 2015 Elsevier B.V.
Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan; Department of Medicinal Chemistry and Research Institute of Pharmaceutical Sciences, University of Mississippi, University, 38677, MS, United States; Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan; Vaccine Research and Development Center, National Health Research Institutes, Miaoli, 35053, Taiwan; Department of Food Science, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan; Department of Life Science, National Taitung University, Taitung, 95002, Taiwan; Department of Food Science, Faculty of Agricultural Technology, University of Brawijaya, Malang, 65145, Indonesia; Research Center for Austronesian Medicine and Agriculture, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan; Research Center for Tropic Agriculture, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan
