A taxonomic study of Bacillus sp. strain S1-13 isolated from Terasi, an Indonesian shrimp paste

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E. Chukeatirote, N. Arfarita, T. Waratrujiwong, A. Kanghae

2016 International Food Research Journal Vol. 23 Issue 6 Article Cited by 0 SDG 14SDG 17 Quartile

Abstract

A bacterial strain S1-13 was screened and isolated from Terasi, a traditional fermented seafood product of Indonesia. This isolate, producing an extracellular protease with high activity when culturing on casein agar, was studied in terms of their phenotypic and genotypic properties including morphology, biochemical profiles, and rRNA gene sequence. The isolate S1-13 was a rod-shaped, endospore-forming, Gram-positive bacterium. Further analysis based on the results of its biochemical profiles and 16S rRNA gene sequence, showed that the strain S1-13 belonged to the B. subtilis / B. amyloliquefaciens group. Additionally, the bacterial isolate S1- 13 was also evaluated for its antibiotic susceptibility and it was found to be susceptible to most antibiotics tested (chloramphenicol, erythromycin, kanamycin, tetracycline and vancomycin), intermediately susceptible to bacitracin, and resistant to ampicillin. © 2016, International Food Research Journal. All Rights Reserved.

Affiliations

School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand; Faculty of Agriculture, Brawijaya University, Malang, 65145, Indonesia

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