Elfi Anis Saati
Rose is a major cut flower in Batu city - East Java Province, Indonesia. Crown of roses has been known to contain anthocyanin pigments from the group of flavonoids. Lately, polyphenolic flavonoid pigment was widely studied due to its high antioxidant power. In vivo studies of antioxidant power in liver were performed through SGOT (Serum Glutamic Oxaloacetic Transaminase) analyses. This study aimed to determine the antioxidant power of anthocyanin pigments contained in the crown of roses. Samples were obtained from rose farmers in the village of Sidomulyo, district of Batu, East Java. Pigment was firstly extracted using distilled water and citric acid solvent. The extract was then filtered using Whatman paper 41 and concentrated in a rotary vacuum evaporator at 50°C. Following fractionation of C18 (Shimadzu chromatograph: acetonitrile 100% and 4% formic acid), isolates obtained were tested in white rats for antioxidant power using activity of SGOT. Results showed that SGOT content of white rats treated with 100% anthocyanin isolates was decreased from 117.542 U/l to 18.267 U/l. This value have approached the normal level of rats without CCl4 injection is 17.075 U/l. © 2006-2016 Asian Research Publishing Network (ARPN).
Department of Science and Food Technology, Faculty of Agriculture and Animal Husbandry, Muhammadiyah University of Malang Campus III UMM, Tlogomas Street, Malang, Indonesia
