Sutrisno Sutrisno, RR Catur Leny Wulandari, Dwi Wahyu Wulan Sulistyowati, Ratna Feti Wulandari, Endang Sri Wahyuni, Yuyun Yueniwati, Sanarto Santoso
Objective: To investigate the effect of genistein on proinflammatory cytokines, NF-κ B activation, and estrogen receptor-β expression in a mice model of endometriosis. Methods: Forty female mice (Mus musculus) were divided into eight groups (n=5 each), including the control (untreated) group, endometriosis group, and the endometriosis groups were given various doses of genistein (at doses of 50; 100; 200; 300; 400; 500 mg/day). Analysis of TNF-α, IL-1β, IL-6, and IL-8 level were done by ELISA technically. Analysis of estrogen receptor-β and NF-κB were done by immunohistochemistry. Results: The level of TNF-α, IL-1β, IL-6, and IL-8 were significantly higher in the EM group compared to the untreated control group (P<0.05). All doses genistein significantly prevented EM-induced increase in TNF-α level (P<0.05), but only at dose of 300 mg/day reach the level in the control group (P>0.05). These increased levels of IL-1β, IL-6, adn IL-8 in the EM group were significantly reduced by all doses of genistein. There were significantly (P<0.05) increased estrogen receptor-β expression and NF-κB activation in EM group compared to untreated group. Only first and fourth dose significantly (P<0.05) decreased the estrogen receptor-β expression compared to the EM group, to reach a level in the control group (P>0.05). All doses genistein significantly prevented EM-induced increase in NF-κB activation (P<0.05), to reach the expression on control group. Conclusions: In conclusion, genistein prohibits the increase in proinflammatory cytokines, NF-κB, and estrogen receptor-β expression in a mice model of endometriosis. © 2015 Hainan Medical College.
Division of Fertility, Endocrinology and Reproduction, Department of Obstetric and Ginaecology, Saiful Anwar General Hospital, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia; Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia; Faculty of Medicine, Sultan Agung University, Semarang, Central of Java, Indonesia; Health Polytechnical of Bangkalan, Health Ministry, Bangkalan, East Java, Indonesia; Pamenang Midwifery Academy, Kediri, East Java, Indonesia; Physiology Molecular Laboratory, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia; Department of Radiology, Saiful Anwar General Hospital, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia; Microbiology Laboratory, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
