Wahyu Widowati, Laura Wijaya, Dian Ratih Laksmitawati, Rahma Micho Widyanto, Pande Putu Erawijantari, Nurul Fauziah, Indra Bachtiar, Ferry Sandra
Endothelial dysfunction in atherosclerosis is associated with increasing oxidative stress that could be reversed by antioxidant. Therefore epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC) and catechin (C) of tea flavonoids were investigated for their roles in regenerating endothelial cell. Peripheral blood mononuclear cells (PB-MNCs) were isolated, plated and cultured in medium with/without treatment of EGCG, ECG, EGC and C. Results showed that among all EGCG, ECG, EGC and C concentrations tested, 12.5 µmol/L was not cytotoxic for peripheral blood-derived endothelial progenitor cells (PB-EPCs). Treatment of EGCG, ECG, EGC or C increased the percentages of CD34, CD133, VEGFR-2 expressions and suppressed hydrogen peroxide-induced percentages of reactive oxygen species (ROS) level in PB-EPCs. Taken together, our current results showed that EGCG, ECG, EGC or C of tea flavonoids could induce differentiation of PB-MNCs into PB-EPCs as well as protect PB-EPCs from oxidative damage by suppresing the intracellular ROS levels. © 2016 Korean Society of Pharmacognosy. All rights reserved.
Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, 40164, Indonesia; Stem Cell and Cancer Institute, Jakarta, 13210, Indonesia; Faculty of Pharmacy, Pancasila University, Jakarta, 12640, Indonesia; Faculty of Agricultural Technology, Brawijaya University, Malang, 65145, Indonesia; Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, 40163, Indonesia; Faculty of Dentistry, Trisakti University, Jakarta, 11440, Indonesia; Prodia Clinical Laboratory, Jakarta, 10430, Indonesia
