Kartini Zailanie, Hartati Kartikaningsih, Umi Kalsum, Yushinta Aristina Sanjaya
In this study, the cytoprotective effects of fucoxanthin were isolated from Sargassum filipendula towards HeLa cell damage which characterized DNA damage. The purpose of this study was to obtain fucoxanthin isolated from S. filipendula and to determine the damage effect of fucoxanthin isolated against HeLa cells by Tunnel method. Fucoxanthin was identified with qualitative methods refer to fucoxanthin Rf 0.25 to 0.28. Fucoxanthin 97% purity was tested using the method of ESI LCMS. We obtained molecular weight of 658.77 m / z. Antioxidant activity in fucoxanthin of S. filipendula was assessed by DPPH for 1.0974 ± 0.0306 ppm with IC50 = 1.4174 ± 0.0126 ppm. While the test of cell apoptosis assay using the TUNEL method with the best dose anti cancer fucoxanthin of 50 ppm and 100 ppm indicates approaching 100% of apoptotic cells. © 2015, Sphinx Knowledge House. All rights reserved.
University of Brawijaya, Jl. Veteran Malang, 65145, Indonesia
