Ni Wayan Manik Parwati, I. Komang Lindayani, Retty Ratnawati, Sri Winarsih, Tatit Nurseta
Objective: To study the possible in vitro effect of tea plant parasite [. Scurrula atropurpurea (Blume) Danser] extract on HeLa cells culture growth inhibition through DNA repair and apoptosis intrinsic pathways mechanism. Methods: Flow cytometry assay were performed for those analyses. Results: For DNA repair pathway mechanism, treatment of tea plant parasite extract decreased MDM2 expression and increased p21 expression. And for apoptosis markers measurement on bax and bcl-2 showed an opposite result, increasing on bax and decreasing on bcl-2 expression. Almost all doses treatment in all measurement showed significantly result. Conclusions: This result showed an effect of tea plant parasite extract on inhibition of cells HeLa culture growth through DNA repair and apoptosis intrinsic pathways mechanism. © 2015 Asian Pacific Tropical Medicine Press.
Magister Program of Obstetrics, Medical Faculty, Brawijaya University, Malang, East Java, Indonesia; Bali Health Academy, Denpasar, Bali, Indonesia; Polytechnic Health Ministry of Health, Denpasar, Bali, Indonesia; Physiology Laboratory, Medical Faculty, Brawijaya University, Malang, East Java, Indonesia; Microbiology Laboratory, Medical Faculty, Brawijaya University, Malang, East Java, Indonesia; Oncology Division, Department of Obstetrics and Gynaecology, Medical Faculty, Brawijaya University, Malang, East Java, Indonesia
