Vivi Shofia, Aulanni’Am Aulanni’Am, Sasangka Prasetyawan
Inflammatory Bowel Disease (IBD) is a chronic inflammatory disease in the gastrointestinal tract, especially the small intestine and the colon. One cause of IBD is a side effect of the use of non steroidal anti-inflammatory drugs (NSAIDs), such as Indomethacin. Indomethacin is not selectively inhibit both COX-1 and COX-2. COX-2 isused to reduce toxicity in the gastrointestinal tract. This study consisted of 2 phases, were study of molecular docking trough in silico studies using COX-2 with Apigenin compound as a ligand contained in methanolic extract of of Atactodea striata. Also in vivo studies of methanolic extract of Atactodea striata theraphy in IBD rats to reduce MDA level. In vivo studies used male rats (Rattus norvegicus), Wistar strain, divided into 4 groups: negative control (healthy rats), positive control (IBD rats) and group IBD with theraphy rats dose of 100 mg/kg BW and 400 mg/kgBW. The Results of in silico studies showed molecular interactions by molecular docking between ligand (Apigenin) and COX-2 (pdb code: 3LN1) resulting the level of bond interaction and visualization of interaction level. It had value of Ki (inhibition constant) of 16.88μM and the value of bond energy to be -6.51 kcal/mol. Statistical analysis indicated that methanolic extract of of Atactodea striata therapy showed significant differences (p <0.05) in reducing of MDA levels on IBD rats. © 2015, Sphinx Knowledge House. All rights reserved.
Laboratory of Biohemistry, Department of Chemistry, Brawijaya University, Indonesia; Brawijaya University, Jl. Veteran, Malang, 65145, East Java, Indonesia
