Mohammad Saifur Rohman, Frastiqa Fahrany, Imama Maslahah, Widodo, Mifetika Lukitasari, Aditya Kurniawan, Agustina Tri Endharti
Renin distal enhancer plays a crucial role in regulating renin gene (REN) expression. REN C-5312T enhancer polymorphism increased enhancer activity. REN C-5434T was also identified which supposed to part of enhancer region. Therefore, this study aimed to investigate contribution of both C-5312T and C-5434T to serum angiotensin I in response to angiotensin II receptor blockers (ARB). C-5312T was identified in 46 hypertensive patients by using multiplex polymerase chain reaction (PCR), while C-5434T by using PCR-restiction fragment length of polymorphism (RFLP). Angiotensin I was measured using enzyme linked immunosorbent assay (ELISA). A significant difference of baseline angiotensin I was observed between -5312CC and - 5312CT/TT (p=0.038) as well as the angiotensin I after 5 months of ARB treatment (p=0.008) in -5434CT/TT population, but not REN C-5434T. In conclusion, C-5312T resulted in increased mRNA renin level as consequence of higher enhancer activity not only at the baseline but also after 5 months ARB treatment. © 2016, Bangladesh Pharmacological Society. All rights reserved.
Department of Cardiology and Vascular Medicine, Faculty of Medicine, University of Brawijaya-Saiful Anwar General Hospital, Malang, 65-145, Indonesia; Biomedical Science, Faculty of Medicine, University of Brawijaya, Malang, 65-145, Indonesia; Brawijaya University Hospital, Malang, 65-145, Indonesia; Biology Department, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, 65-145, Indonesia; Nursing Science, Faculty of Medicine, University of Brawijaya, Malang, 65-145, Indonesia; Parasitology Department, Faculty of Medicine, University of Brawijaya, Malang, 65-145, Indonesia
