Dharma P.T.R. Maluegha, M. Aris Widodo, Bambang Pardjianto, Edi Widjajanto
This study was aimed to investigate the effects of bromelain on angiogenesis, nitric oxide, and matrix metalloproteinase-3 and -9 in rats exposed to 1200mV electrical burn injury. Thirty-five, male Wistar albino rats was divided into five groups (n=7 each), including control (untreated) group; electrical burn injury (EI) group; electrical burn injury+21.25mg/kg BW bromelain group (EIB1); electrical burn injury+42.50mg/kg BW bromelain group (EIB2); and electrical burn injury+85.00mg/kg BW bromelain group (EIB3). Rat models of electrical burns done by providing electricity in rats that had been anesthetized, a voltage of 1200mV and strong currents 15mA for 10s. The VEFG, NO, and MMP-9 levels were significantly greater in the EI group compared to the untreated group. Out of the 21.25mg/kg BW, 42.50mg/kg BW, and 85mg/kg BW doses of Bromelain extract, only the lowest doses prevented EI-induced increase in NO and MMP-9 level (P<0.05). Bromelain at the lowest dose (21.25mg/kg BW) act as anti-inflammatory and modulate the matrix metalloproteinase-9 in rats exposed to 1200mV electrical burn injury. © 2015 Elsevier GmbH.
Biomedical Sciences Doctoral Study Program, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia; Pharmacology Laboratory, Faculty of Medicine, University of Brawijaya, Malang, East Java, Indonesia; Department of Plastic Surgery, Saiful Anwar General Hospital, Faculty of Medicine, University of Brawijaya, Malang, East Java, Indonesia; Clinical Pathology Laboratory, Saiful Anwar General Hospital, Faculty of Medicine, University of Brawijaya, Malang, East Java, Indonesia
