Umar Paputungan, Luqman Hakim, Gatot Ciptadi, Hapry F. N. Lapian
This study was done to evaluate live weight performance of different growth hormone (GH) genotypes (BC+/+, BC+/–, BC–/–) of progeny generation (G1) derived from parental generation (G0) of three genotypes in cows (C+/+, C+/–, C– /–) and two homozygous genotypes in sires (B+/+ and B–/ –) mated by artificial insemination (AI) in Indonesian-grade cattle at rural areas in Minahasa regency, North Sulawesi province of Indonesia. To indentify the presence of GH genotypes, the totals of 222 blood samples of Indonesian-grade cows (G0) and their female progeny (G1) as well as 2 blood samples of Ongole-breed sires (G0) were used in this study by PCR-RFLP involving Msp1 enzyme-restriction. To eliminate different age effects of the animals, live weight data of 111 G1 progeny were adjusted for the 50 and 345 days old of ages for the first and second weighing, respectively. Cows and their progeny grazed onto places within local grass pasture all days starting at 07.00 a.m. to 17.30 p.m. without supplementary feeds of concentrate as the main management system practised by 116 farmers as the animal owners. The breeding herds were on the range pasture year around. Farmers supervised their cows and the animals showing signs of estrus were brought at the rural AI service center of government to be mated freely by AI inseminator using thawing straw of frozen germ plasma of Ongole-breed sires in liquid Nitrogen. Data on live weight and average daily gain (ADG) were recorded on individual animal basis of different GH genotypes and analyzed using general linear models. The results showed that GH genotypes were associated with differences on live weight and ADG of Indonesian-grade progeny (G1) during 50 to 345 days of age. The heterozygous GH genotype (BC+/-) excelled over their homozygous GH genotypes (BC+/+ and BC-/-) in respects of progeny live weight gain. It is concluded, the homozygous GH genotypes of sire (B+/+, B– /–) and cow (C+/+, C–/–) and the heterozygous GH genotype of cow (C+/–) can be used as the potential GH genotypes in Indonesian grade cattle for progeny live weight gain improvement at rural areas. © 2016, Fundacion CIPAV. All right reserved.
Sam Ratulangi University, Manado, 95115, Indonesia; Brawijaya University, Malang, 65145, Indonesia
