Herlinda Mawardika, Suharjono
One of the problems in tomato cultivation was wilt disease caused by pathogenic Fusarium. The effective solution was using antagonist molds as biological control agent against pathogenic Fusarium. The purposes of this research were to determine potency of antagonist molds and to identify potential antagonist mold to inhibit growth of pathogenic Fusarium. Pathogenic Fusarium were isolated from tomato stems, while antagonist molds were isolated from soil sample. Screening of isolates and antagonist assay was carried out using dual culture method, then continued with non-volatile metabolite assay. Percentage of inhibition zone was analyzed by One-way ANOVA at a significant level of α = 0.05 by SPSS. Mold isolates were identified based on Internal Transcribed Spacer (ITS). Isolate FB.1 and FB.2 were obtained from tomato stems. Three soil molds, KT.7, KT.10, and KT.16 have antagonistic activity to pathogenic Fusarium. KT.16 colony has the highest antagonistic activity against isolate FB.1 (59.84 %), whereas isolate KT.10 against isolate FB.2 (54.67 %). Metabolites of isolate KT.16 showed the highest inhibition percentage against FB.1 and FB.2 isolates (34.82 and 33.67 %). Isolate KT.16 was the best antagonist mold and was identified as Trichoderma longibrachiatum ATCC 52326 with similarity 100 %. © 2015, Sphinx Knowledge House. All rights reserved.
Department of Biology, University of Brawijaya, Malang, Indonesia
