Eva Watamtin Widhiya, Suharjono, Tri Ardiyati
The chemical fertilizers cause a decrease in soil fertility and productivity of apple crops. Rhizosphere bacteria may be used as a biofertilizer agents due to its ability to secret produce metabolites required for the growth of plants. Three bacteria isolate SL4, SL5, and SL7 of apple crops rhizosphere (Apple crops in Batu, Indonesia), were observed through PGP (Plant Growth Promoting) activity traits that consist of cellulose activity, phosphate solubilization, and nitrogen fixation. Quantitative IAA production of three isolates was evaluated, and molecular identification base on 16S rDNA was conducted by MEGA 6.0 software. Results showed IAA concentration of consortium culture highest than the single culture of the isolate is 3,42 μg/mL after 5 days incubation. Bacterial isolates identification showed that SL4 has similarity 99,82% as Bacillus subtilis F3-7, SL5 has similarity 99,43% as Staphylococcus arlettae 5H7a, and SL7 has similarity 99,94% as Bacillus methylotrophicus CL12. © 2016, Sphinx Knowledge House. All rights reserved.
Laboratory of Microbiology, Biology Department, Faculty of Mathemathics and Natural Science Brawijaya University, Malang, Indonesia
