Ninis Trisyani, Endang Yuli Herawati, Maheno Sri Widodo, Daduk Setyohadi
Solen sp. are clam species from Phylum Mollusca with potential economic value. DNA Barcode uses the cytochrome-C oxidase subunit I (COI) gene to provide an alternative method for species identification. The aim of this study was to evaluate the genetic relationships among razor clam species (Solen sp.) in the coastal areas of Surabaya and Pamekasan, using the COI DNA Barcode. The razor clam genome was isolated, and the COI gene was amplified using a universal primer. Thus sequences were analyzed for genetic variation and phylogenetic comparison. The results of alignment showed 43 substitutions of nucleotide bases, which consisted of 37 transitions and 6 transversions with no insertions or deletions. The phylogenetic tree was obtained using the Neighbor Joining and Minimum Evolution methods in combination with Kimura Model 2. The results showed Solen sp. of Surabaya have a divergent sequence of 3.1% with similarity of 96.86% in comparison to Solen regularis. Solen sp. of Pamekasan also clustered with Solen r., with genetic distance of 17.3% and 82.69% similarity. This novel Solen sp. barcode will help provide a database for Indonesian Bivalvia, to be recorded using the Barcoding of Life data system. © 2016, BIOFLUX SRL. All rights reserved.
University of Brawijaya, Malang, Indonesia; University of Hang Tuah, Faculty of Engineering and Marine Science, Department of Fisheries, East Java, Jalan Arif Rahman Hakim 150, Surabaya, 60111, Indonesia; Faculty of Fisheries and Marine Sciences, University of Brawijaya, Department of Aquatic Resource Management, East Java, Malang, 65145, Jalan Veteran, Indonesia
