Nurdiana Nurdiana, Nelly Mariati, Noorhamdani Noorhamdani, Bambang Setiawan, Nicolaas Budhiparama, Zairin Noor
Objective To analyze the effects of Labisia pumila (L. pumila) extract on the markers of oxidative stress in ovariectomized rats. Methods Twenty-five female Wistar rats were divided into five treatment groups (n = 5): the control group, the ovariectomized group, the ovariectomized group treated with L. pumila extract of various doses (10 mg/kg, 20 mg/kg, and 40 mg/kg). L. pumila extract was administered daily for 8 weeks. Results The levels of malondialdehyde and superoxide dismutase (SOD) were subjected to spectrophotometric analysis. Serum levels of malondialdehyde and SOD were significantly higher in the group of rat model of post-menopausal osteoporosis than that of the control group (P < 0.05). All doses of L. pumila administered significantly decreased the serum levels of malondialdehyde relative to the group of rat model of post-menopausal osteoporosis, reaching the levels comparable to those of the control group (P < 0.05). The second dose of L. pumila significantly decreased the levels of SOD relative to those of the group of rat model of post-menopausal osteoporosis, although it did not reach the levels of SOD of the control group (P < 0.05). Conclusion The ethanol extract of L. pumila normalizes lipid peroxidation in the rat model of post-menopausal osteoporosis via the mechanism of endogenous antioxidant replacement. © 2016 Hainan Medical College
Department of Pharmacology, Faculty of Medicine, University of Brawijaya, Malang, East Java, Indonesia; Midwifery Master Study Programme, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia; Department of Microbiology, Faculty of Medicine, University of Brawijaya, Malang, East Java, Indonesia; Research Center for Toxicology, Cancer, and Regenerative Medicine, Department of Medical Chemistry and Biochemistry, Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia; Budhiparama Institute of Hip and Knee Research and Education Foundation for Arthroplasty, Sports Medicine and Osteoporosis, Jakarta, Indonesia; Research Center for Osteoporosis, Department of Orthopaedic and Traumatology, Ulin General Hospital, Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
