Gatot Ciptadi, Ali Harris, Helly Nurul Karima, Setyawati
The aims of this research were to evaluate in vitro maturation of oocytes from prepubertal goats obtained from a local slaughterhouse. In this work oocytes were matured in vitro to determine the effect supplementation of Estrus Goat Serum (EGS) in TCM 199. The immature goat oocytes were aspirated from follicles of 2.0 to 6.0 mm in diameter using 10 ml syringe and 18G needle. Oocytes were matured for 26 hours at 39oC and 5% CO2 with maximum humidity. Results showed that the percentage of cumulus expansion were 32,7% (TCM 199), 80,1% (TCM+10% EGS), 81,8% (TCM+15% EGS) and 83,1% (TCM+20% EGS) respectively. Meanwhile, the percentage of nuclear maturation of M-II were 26,5%, 61,2%, 80,8%, and 81,3%. The percentage of nuclear maturation (M-II) was also significantly different (p < 0.05). The level of EGS supplementation resulted in significantly different maturation rate. As well as the EGS concentration increased, the higher maturation rate was also observed. The highest maturation were obtained with 15% and 20 % EGS, but the difference is not significant. It was concluded that immature oocytes from prepubertal goat supplemented with EGS were a significant effect to their maturation rate, both base on cumulus expansion and nuclear maturation.
Department of Animal Production, Faculty of Animal Husbandry, Brawijaya University, Malang, 65145, Indonesia; Faculty of Fishery, Mataram '45, University, Mataram, 83239, Indonesia; Biology Department of FMIPA-UB, Brawijaya University, Malang, 65145, Indonesia; Laboratorium of Life Sciences, LSIH-UB, Brawijaya University, Malang, 65145, Indonesia
