I. Muhaimin Rifa, Yuyun Ika Christina, Qonitatul Khasanah, Agung Pramana Warih Marhendra, Hideo Tsuboi
Inflammation is an immunological response which plays pivotal role in antigen elimination, but it also causes detrimental effect to the surrounding tissue. Inflammation pathway is activated by the molecular signaling which regulate both pro- and anti-inflammatory cytokines. NF-κB is a transcription factor which regulates the transcription of several genes that regulate growth, angiogenesis, and survival, including the transcription of inflammatory cytokines. In the present study, we examined the effect of polyherbal EMSA Eritin focusing on immunological function with aim of highlighting the production of pro-inflammatory cytokines and the expression of transcriptional factor NF-κB in vitro. EMSA Eritin is a polyherbal consisted of soybean, red rice, and coconut water extract. Splenocytes from healthy mice were cultured for 5 days in RPMI-1640 medium. On day 5, the cultured cells were harvested and analyzed by flow cytometry. EMSA Eritin was able to inhibit the expression of NF-κB in T cells and also the production of proinflammatory cytokines marked by the decreasing levels of CD4+IFN-γ+ and CD4+TNF-α+ T cells. Previous study indicated that EMSA Eritin is able to decrease NF-κB and pro-inflammatory cytokines in irradiated mice (in vivo study) with sublethal dose. Hereby, our study suggests that EMSA Eritin is a potential herbal medicine which act as an anti-inflammatory agent. © 2016, International Journal of Pharmaceutical and Clinical Research. All Rights Reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia; Faculty of Veterinary Medicine, Brawijaya University, Malang, Indonesia; Department of Immunology, Nagoya University Graduate School of Medicine, Nagoya, Japan
