Suhargo Listijani, S.B. Sumitro, Widodo
Dipeptidyl Peptidase-4 (DPP-4) is an epithelial transmembrane protein of many cell types such as gastrointestinal tract, biliary tract, pancreas, kidney, thymus, uterus, prostate and capillary endothelial cells. DPP-4 also found in soluble form of serum and other body fluid. Soluble DPP-4 has not a cytoplasmic domain and transmembrane domain. In diabetes, hydrogen peroxide as oxidant was involved in soluble DPP-4 generation from transmembrane DPP-4. In Benign Prostatic Hyperplasia (BPH), there were decreasing of antioxidant such as superoxide dismutase, catalase and gluthathione peroxidase, so the antioxidants in those diseases unable to discharge the oxidants and there was an accumulation of oxidants. This study aims to evaluate the role of some oxidant on DPP-4 enzyme in BPH through computational approach. The oxidants such as H2O2, OH-, HOCl, NO-, NO2 ONOO-, 13-hydroperoxy linoleic acid and 13-hydroxy linoleic acid (13-HODE) were docked with dipeptidyl peptidase-4. The result showed that all the oxidants may interact with DPP-4 on hydrolase domain (Gln 508 – Pro 766) or on β-propeller domain (Arg 54 - Asn 497). But 13-HODE may interact with DPP-4 on the stalk of DPP-4 that were on Asp 34, Ala 35, Thr 36, dan Ala 37 residues. So it may cause DPP-4 shedding to produce soluble DPP-4. © 2016, International Journal of Pharmaceutical and Clinical Research. All Rights Reserved.
Department of Biology, Faculty of Mathematics and Natural Science, Brawijaya University, Malang, East Java, Indonesia
