Muharfiza, Dimas Firmanda Al Riza, Yoshito Saito, Kenta Itakura, Yasushi Kohno, Tetsuhito Suzuki, Makoto Kuramoto, Naoshi Kondo
Monitoring the maturation process of Satsuma mandarin (Citrus unshiu Marc.) by determining the soluble solids (SS) and acid content non-destructively is needed. Fluorescence components potentially offer such means of accessing fruit maturity characteristics in the orchard. The aim of this study was to determine the potential of fluorescence spectroscopy for monitoring the stage of citrus maturity. Four major fluorescent components in peel and/or flesh were found including chlorophyll-a (excitation (Ex) 410 nm, emission (Em) 675 nm) and chlorophyll-b (Ex 460 nm, Em 650 nm),polymethoxyflavones (PMFs) (Ex 260 nm and 370 nm, Em 540 nm), coumarin (Ex 330 nm, Em 400 nm), and a tryptophan-like compound (Ex 260 nm, Em 330 nm). Our results indicated a significant (R2 = 0.9554) logarithmic ratio between tryptophan-like compoundsExEm and chlorophyll-aExEm with the SS:acid ratio. Also, the log of the ratio of PMFs from the peel (ExExEm was significantly correlated with the SS:acid ratio (R2 = 0.8207). While the latter correlation was not as strong as the former, it does demonstrate the opportunity to develop a non-destructive field measurement of fluorescent peel compounds as an indirect index of fruit maturity. © 2017 by the authors.
Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan; Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture, Jakarta, 12540, Indonesia; Department of Agricultural Engineering, Faculty of Agricultural Technology, University of Brawijaya, Jl. Veteran, Malang, 65145, Indonesia; Department of Planning and Environment, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime, 700-2405, Japan; Advanced Research Support Center, Ehime University, 2-5 Bunkyo-cho, Matsuyama, Ehime, 790-8577, Japan