Ni Komang Erny Astiti, Dra. Sri Winarsih, Tatit Nurseta, Moch. Muljohadi Ali, Gusti Ayu Marhaeni, I Komang Lindayani
Background: Cervical cancer is one of infectious diseases caused by HPV. Its medical treatment cause negative affect on healthy cells and increase the risk of new cancer formation. Therefore some plants are known to be potential as medicine for cancer treatment. Aim: Here we aimed to determine the effect of the chloroform fraction of epiphyte tea leaf extract (TELECF, 25, 50, 100, 200, and 400 μg/ml) on caspase 9 and caspase 3 expression in cultured HeLa cells. Materials and Methods: HeLa cells was cultured for six experimental groups and four replication. Caspase 3 and caspase 9 expression on HeLa cells were monitored by flow cytometry. One-Way Analysis of Variance was used for data analysis. Results: We found that TELECF increases caspase 9 and caspase 3 expression in HeLa cells. The highest caspase 9 expression level (73.728% ± 3.019) was detected with 25 μg/ml TELECF. The highest caspase 3 expression level (66.953% ± 2.367) was detected with 50 μg/ml TELECF. Conclusion: In general, the result indicate that TELECF can increases Caspase 9 and Caspase 3 expression in HeLa cells. © 2018 Wolters Kluwer Medknow Publications. All rights reserved.
Midwifery Department, Polythechnic of Health, DENPASAR, Indonesia; Microbiology Laboratory, Faculty of Medicine, Brawijaya University, Indonesia; Department of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University, Division of Fertility Reproductive Endocrinology, Indonesia; Microbiology Laboratory, Faculty of Medicine, India
