Kumboyono Kumboyono, Indah Nur Chomsy, Wahyu Santoso Hidayat, Ardhi Khoirul Hakim, Hidayat Sujuti, Titin Andri Wihastuti
Introduction: Oxidative stress describes an imbalance of pro-oxidant and antioxidant levels in cells. This study aimed to investigate the significant correlation of Cotinine on Monocyte Chemotactic Protein-1 (MCP-1) and Nitric Oxide (NO) as an indicator of vascular function. Methods: The research, designed as a comprehensive cross-sectional study, included a sample of non-smokers (n=100) and smokers (n=100). The study utilised Cotinine, MCP-1 Human MCP-1 ELISA kit, and Nitric Oxide Colorimetric Assay kit, with data analysis conducted using Wrap Partial Least Square. Results: This study showed a significant effect between Cotinine with MCP-1 (p-value <0.001) and Cotinine with NO (p-value <0.029). The impact of MCP-1 on NO resulted in a p-value of <0.001. Indirectly, it can be known that the effect of Cotinine on NO through MCP-1 produces a p-value of <0.001. The study concluded a significant direct association between Cotinine and MCP-1, Cotinine and NO, and MCP-1 and NO. Meanwhile, the indirect relationship of Cotinine on NO through MCP-1 is paramount. Conclusion: These findings underscore Cotinine's direct and indirect results on vascular function decline, contributing significantly to our understanding of vascular inflammation and providing a new perspective on the impact of smoking on cardiovascular health. © 2025, Societe Tunisienne des Sciences Medicales. All rights reserved.
School of Nursing, Faculty of Health Sciences, Universitas Brawijaya, Indonesia; Biomedical Science Study Program, Faculty of Medicine, Universitas Udayana, Bali, 80232, Indonesia; Master Program of Biomedical Science, Medical Faculty, Universitas Brawijaya, Indonesia; Medical Laboratory Technology Department, Stikes Banyuwangi, Banyuwangi, Indonesia; Biochemistry Department, Medical Faculty, Universitas Brawijaya-Saiful Anwar General Hospital, Indonesia; Basic Nursing Department, Faculty of Health Sciences, Universitas Brawijaya, Indonesia
