Hary Nugroho, Woro Busono, Sucik Maylinda
This research aims to develop a method for selection based on myostatin gene markers. The experiment was conducted in Bali cattle. Blood samples were collected from 100 cattle irrespective of age. PCR-RFLP was applied with primers for amplifying the 1346bp of the Myostatin gene were (F) 5'-CCCTACAGAGGCCACTTCAA-3' and (R) 5'- CTCGCTGTTCTCATTCAGATC-3', sand HaeIII restriction enzymes. PCR-RFLP results showed that not all genotypes present in the population, despite populations that were observed quite polymorphic (PIC = 49.5%) with the conditions of gene frequencies. The results showed that almost all variables influenced by myostatin genotype where the genotype BB is better, except the chest girth showed a better result in genotype AB. It suggested that MSTN is strong candidate gene that influenced growth traits. Moreover, it is also expected that this MSTN could be used in marker-assisted selection. © 2017, Agricultural Research Communication Centre. All rights reserved.
Departement of Animal Production, Faculty of Animal Husbandry, University of Brawijaya, Malang, Indonesia
