Muhammad Sasmito Djati, Yustika Aulia Rahma, Dinia Rizqi Dwijayanti, Muhaimin Rifai, Sri Rahayu
Purpose: To investigate the efficacy of Elephantopus scaber L. and Sauropus androgynus (L.) merr formulations in prolactin and erythrocyte cell production in pregnant typhoid mice. Methods: In the experiment, 21 pregnant BALB/c mice, divided into seven treatment groups (n = 3) were used. All groups, except control group (T1), were orally infected with Salmonella typhi (107 CFU/mL). Individual initial doses of 200 mg/kg E. scaber and 150 mg/kg S. androgynous were given to the mice. Extracts of E. scaber and S. androgynus were then administered orally in the following formulation ratios: 100:0 (T3), 75:25 (T4), 50:50 (T5), 25:75 (T6) and 0:100 % (T7). Blood was isolated from the orbital veins of pregnant mice at 4, 8 and 12 days post-infection, and centrifuged at 2500 rpm and 10 ºC for 5 min. The supernatant was separated from the pellets to obtain the serum. Hematopoietic cells were isolated from bone marrow at 12 days post-infection. Prolactin hormone level was determined by enzyme-linked immunosorbent assay (ELISA), and erythrocytes were measured by fluorescence-activated cell sorting (FACS). Result: Salmonella typhi infection in pregnant mice reduces levels of prolactin and TER119+ cells. T7 formulation increased (p < 0.05) prolactin levels on days 8 (3.83 ± 0.93 ng/mL) and 12 (3.45 ± 0.39 ng/mL) post-infection. Furthermore, the T3, T5, and T7 formulation may have elevated the number of TER119+ cells compared to the control group. Mice given the T3 formulation showed increased numbers of TER119+ VLA4+ (68,73 %), those given T5 showed comparable numbers of TER119+ VLA4-(45.81 %) and TER119+ VLA4+ (54.19%), while those given T7 showed increased the numbers of TER119+ VLA4- (97,45 %). Conclusion: E. scaber and S. androgynus leaves extracts significantly increased (p < 0.05) the levels of prolactin and erythrocytes to support the pregnancy of BALB/c mice with typhoid model. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.
Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Veteran Street, Malang, 65145, East Java, Indonesia
