Happy Nursyam, Sri Andayani, Affandi Saputra
The consequences of blood biochemistry changes in fish which was exposed to tannin compound (poliphenol) have not been investigated. Furthermore, the impact of plant decomposition in certain water is poorly understood. North African catfish (Clarias gariepinus) was injected with a single dose of Piper crocatum tannin. The fish was exposed (LD 25, 50 and 75% from 96 hours LC50) to tannin concentration with the dose of 0.36, 1.08 and 1.80 mg/kg, and (75% of 96-hour LC50) to phenol concentration (3 mg/kg) and P. crocatum tannin (1.8 mg/kg). The effects of tannin on hemoglobin and blood biochemistry were measured 3 days after injection. The erythrocyte on hemoglobin and hematocrit decreased significantly. The blood biochemistry such ALP (alkaline phospatase) and glucose also significantly decreased. Meanwhile, there were some increase in blood urea nitrogen (BUN) and cortisol hormone. The histopathological analysis of fish kidney and liver after phenolic exposure indicated some tissue damage such as hypertrophy, congestion, and necrosis. This is the first report on effect of P. crocatum leaves tannin on blood biochemistry and histopathology. © 2017, BIOFLUX SRL. All rights reserved.
Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, 65145, Indonesia; Campus BAPPL, Institute of Fisheries Science, Jl. Raya Karangantu, Serang, 42191, Banten, Indonesia
