Fadjar Kurnia Hartati, Simon Bambang Widjanarko, Tri Dewanti Widyaningsih, Muhaimin Rifa’i
Black rice has been known for its many beneficial effects to our body because it contains several chemical compounds that act as anti-inflammatory agents. This study aims to compare the anti-inflammatory activity of ethanol extract and aqueous extract of black rice. Anti-inflammatory activity test was performed in vitro on splenocytes of diabetes mellitus mice model as the control of inflammation. Cells were cultured in Roswell Park Memorial Institute medium with 10% fetal bovine serum, anti-CD3 and lipopolysaccharide and black rice extract 50, 100 and 200 µg/mL. In day 3, cells were harvested, stained and analyzed by flow cytometry. The result shows that the ethanol extract and aqueous extract of black rice have anti-inflammatory activity. 50 µg/mL black rice aqueous extract has the most extensive anti-inflammatory activity indicated by increased Treg cells, decreased nuclear factor kappa B activity on CD4+, CD8+ T cells, decreased production of TNF-α by CD4+ T cells, decreased production of IL-6 and IFN-γ by macrophages. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
Post Graduate Program, Faculty of Agricultural, Brawijaya University, Malang, Indonesia; Department of Food Technology, Faculty of Agricultural, Dr. Soetomo University, Surabaya, Indonesia; Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Malang, Indonesia; Biology Department, Faculty of Mathematic and Natural Sciences, Brawijaya University, Malang, Indonesia
