Fitra Arya Dwi Nugraha, Kholifah Holil, Nia Kurniawan
Ecological damages to the Lagoon of Segara Anakan, Central Java, as well as large-scale and continuous exploitation are threatening the sustainability of fine shrimp, Metapenaeus elegans, and resources. Information in regards to genetic resources is crucial to establish long-term conservation programs and to preserve germplasm quality. This study aims to evaluate the number and size of the fragment which is digested with restriction enzyme Hind III. Seven individuals of Metapenaeus elegans from the Lagoon of Segara Anakan were examined using Hind III. Amplification of mitochondrial DNA resulted in 950 bp, and the digestion using Hind III generated four fragments consisting of 114 bp, 200 bp, 250 bp, and 386 bp, which formed a monomorphic pattern. The restriction pattern showed the probability of homozygosity of alleles that restricted using Hind III. Homozygosity indicates no variation of DNA sequence. © 2017 Author(s).
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, East-Java, Indonesia; Department of Biology, Faculty of Science and Technology, State Islamic University of Maulana Malik Ibrahim, East-Java, Indonesia
