Ovi Sofia, Damaryati Widyastuti, L. Hidayat Sujuti, Karyono Mintaroem
Background To compare the aqueous levels of interleukin-6 (IL-6) in endotoxin-induced-uveitis (EIU) rat models after topical administration of 1% prednisolone acetate, 0.1% nepafenac or 0.1% diclofenac. Methods EIU was induced in rats by a footpad injection of 200 μg lipopolysaccharide (LPS). The animals were divided into five groups: negative control, positive control, LPS + 1% prednisolone acetate, LPS + 0.1% nepafenac and LPS + 0.1% diclofenac. One hour after LPS administration, 1% prednisolone acetate, 0.1% nepafenac or 0.1% diclofenac was topically applied to both eyes of rats and repeated every six hours. After 20 hours, rats were anesthetized and 10 μl aqueous humor was sampled, followed by measurement of IL-6 levels by ELISA method. Results In this study, there was a significant difference in aqueous levels of IL-6 between treatment groups (p < 0.05). The lowest levels of IL-6 were obtained in the 1% prednisolone acetate group (32.5040 ± 4.5839 pg/ml), which was similar to the negative control (23.2760 ± 6.3958 pg/ml). The highest mean was the positive control (109.9426 ± 8.5629 pg/ml). The mean of IL-6 in the 0.1% nepafenac group was 50.1971 ± 3.7171 pg/ml, higher than in the 0.1% diclofenac group (59.2200 ± 18.1651 pg/ml), but not statistically significant. Conclusion There was a significant difference in aqueous levels of IL-6 in EIU rat models after administration of 1% prednisolone acetate, 0.1% nepafenac and 0.1% diclofenac, with the highest reduction in IL-6 levels noted in the 1% prednisolone acetate group. © 2018 Wolters Kluwer Medknow Publications. All rights reserved.
Department of Opthamology, Brawijaya University, Saiful Anwar Hospital Malang, East Java, Indonesia
