Yus Isnainita Wahyu, Simon Bambang Widjanarko
This study aims to determine the collagen from extraction optimization of milkfish scale collagen based on the yield and hydroxyproline content due to the treatment of CH3COOH concentration and extraction time. [Method] This research used Response Surface Methodology (RSM) and Central Composite Design (CCD) with independent variable of extraction time(X1, hour) and concentration of acetic acid (X2, M), while dependent variables were yield (Y1,%) and hydroxyproline content (Y2,%). Design Expert software was used for this design. [Result] The result shows that the optimal condition of collagen extraction from milkfish scale is obtained by extraction time of 61.30 hours with CH3COOH concentration of 0.66M, resulting in a yield of 0.737% ± 0.012 and hydroxyproline content of 7.223% ± 0.157, the difference in predictive value and Design Expert is less than 5%. [Conclusion] Result of FTIR, DSC and analysis amino acid show that milkfish scale collagen in this study resemble those of commercial collagen, thus it can be used as alternative source of collagen. © 2018 North University of Baia Mare.
Department of Agricultural Product Technology, Faculty of Agricultural Technology, Brawijaya University, Indonesia; Polytechnic Marine and Fisheries Sidoarjo, Indonesia
