Yunita Diyah Safitri, Mochammad Fitri Atho'illah, Farida Dewi Nur'aini, Sri Widyarti, Muhaimin Rifa'i
High Fat-Fructose Diet (HFFD) triggers various metabolic problems including obesity. Obesity leads to a chronic low-grade inflammation and is associated with the unbalanced production of hematopoietic cells. In the condition of obesity, hematopoietic stem cells (HSC) are more likely to differentiate into granulocytes rather than erythrocytes as a response to inflammation. Modified soybeans are well-known to have higher compounds than the raw soybeans. One of the soybean modification processes is known as elicitation. In this study, soybeans are exposed to stressors such as Saccharomyces cerevisiae and light to increase the beneficial compounds inside. The elicited soybean extract (ESE) contains several anti-inflammatory and anti-oxidant compounds which are useful for the hematopoietic system. This study is aimed at determining the effects of ESE on hematopoietic cells; erythrocytes lineage (TER-119+CD34+, TER-119+VLA-4+, TER-119+), and granulocytes lineage (Gr-1+). In this study, twenty-eight three-week old female Balb/C mice were used. They were fed HFFD for twenty weeks, and were given ESE oral treatment for four weeks. The level of hematopoietic cells was analyzed using flow cytometry. The present study found that HFFD decreased the level of TER-119+CD34+, TER-119+, and TER-119+VLA-4+ level, and increased the Gr-1+ level. ESE significantly increased the TER-119+CD34+, TER-119+ and TER-119+VLA-4+ level (p < 0.05) and decreased the Gr-1+ level (p < 0.05) in the HFFD-treatment group. These results show that the potential use of ESE as an anti-inflammatory agent can improve the hematopoietic system in the HFFD-diet mice model. © 2018 Hashemite University.
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, 65145, Indonesia
