Agustina Tri Endharti, Eni Sulastri, Rabiah Umanailo, Yunialce, Tatit Nurseta, Kusworini Handono
Mango mistletoe (Dendrophthoe pentandra L. Miq) (MMDP) leaf extract which contained quercetin inhibits cancer cells. MMDP increasing effectively of 5-Fluorouracil (5-FU) as a chemotherapy agent. This study aimed to investigate the synergistic effects of MMDP combined with 5-FU to determine the percentage of apoptosis, p21, and survivin in HeLa cells. Flow cytometry analysis revealed that it increases apoptosis HeLa cell through increasing p21 and also decreased survivin percentage. The combination of 5-FU 5 μg/ml and MMDP 50 μg/ml significantly increased the percentage of p21 and apoptosis (P < 0.001; P < 0.001) and decreased survivin (P < 0.05) compared with 5-FU only group. This study indicates that the combined 5-FU and MMDP has a synergistic effect to enhance apoptosis and p21 expressions by decreasing of survivin in HeLa cells. © 2018 Agustina Tri Endharti et al.
Department of Parasitology, Faculty of Medicine, Brawijaya University, Malang, Indonesia; Biomedical Central Laboratory, Faculty of Medicine, Brawijaya University, Indonesia; Faculty of Medicine, Brawijaya University, Malang, Indonesia; Departement of Gynecology and Onco-gynecology Saiful Anwar Hospital, Medical Faculty, University of Brawijaya, Malang, East Java, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Brawijaya University, Malang, Indonesia
