Lilik Indahwati, Linda R. Wati, Sutrisno Sutrisno, Noorhamdani Noorhamdani
Objectives: This study investigated the effects of a human ZP3 monoclonal antibody (mAb hZP3) on the expression of growth differentiation factor-9 (GDF-9) and number of theca cells in the ovaries of mice (Mus musculus). Methods: Our study employed a true experiment posttest-only control group design of 48 mice that were divided into the control and three mAb hZP3-treatment groups (20, 40, and 60 μg). Mice in each group were terminated on days 10, 15, and 20. GDF-9 expression was measured by immunohistochemistry and the number of theca cells was counted. Results: Analysis of the effects of mAb hZP3 (at 20–60 μg) on the expression of GDF-9 and amount of theca cells did not show significant differences. Similar findings were observed throughout the study period (at 10–20 days). Therefore, mAb hZP3 had no effect on GDF-9 expression and theca cells. Conclusion: This study showed that mAb hZP3 can be considered to be an effective and safe immunocontraception. © 2018 The Authors
Department of Midwifery, Faculty of Medicine, Brawijaya University, East Java, Indonesia; Faculty of Medicine, Brawijaya University, Jalan Veteran Malang, East Java, Indonesia; Department of Microbiology, Faculty of Medicine, Brawijaya University, Jalan Veteran Malang, East Java, Indonesia
