Aditya Indra Mahendra, Jonny Karunia Fajar, Harapan Harapan, Teuku Heriansyah, Sumarno Reto Prawiro, Edi Widjajanto, Mohammad Saifur Rohman, Karyono Mintaroem, Budi Susetio Pikir, Yash Prashar
Background: Recently, atherosclerosis-associated disease has been reported simultaneously increased. Whereas, to date, no atherosclerosis vaccine is available. Since the epitope mimicry between malondialdehyde low-density lipoprotein (MDA-LDL) and arginine specific epitope gingipain (Rgp) on the Porphyromonas gingivalis vesicles has been reported, it raises an opportunity to employ the potency of P. gingivalis as an atherosclerosis vaccine. Objective: To evaluate the potency of P. gingivalis vesicles to prevent atherosclerosis, by assessing MDA-LDL level, visceral fat, body weight, and aortic wall thickness, in rats model. Methods: Five groups of rats (n = 10 per group), three treatment groups, one positive and negative control group were assigned and adapted with high fat diet for 8 weeks. The treatment groups were injected with P. gingivalis vesicles with and without adjuvant with four booster doses. The level of MDA-LDL serum, visceral fat, body weight, and aortic wall thickness were measured in the end of the course. Results: Our present study found that decreased in MDA-LDL levels (p = 0.037) and aortic wall thickness (p = 0.016) were observed in rats treated with vesicles and adjuvants, but not with vesicles or adjuvants only, compared to negative control. Moreover, MDA-LDL levels in rats immunized with vesicles and adjuvants were significantly lower than healthy rats. However, body weight (p = 0.329 and visceral fat (p = 0.789) were not significantly different in all treatment groups compared to control. Conclusions: Immunization with P. gingivalis vesicles and adjuvants significantly reduces MDA-LDL level and aortic wall thickness in rats model. © 2018 Association for Research into Arterial Structure and Physiology
Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia; Brawijaya Cardiovascular Research Center, Brawijaya University, Malang, 65145, Indonesia; Medical Research Unit, School of Medicine, Syiah Kuala University, Banda Aceh, 23111, Indonesia; Department of Cardiology and Vascular Medicine, School of Medicine, Syiah Kuala University, Banda Aceh, 23111, Indonesia; Department of Microbiology, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia; Department of Cardiology and Vascular Medicine, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia; Department of Anatomic Pathology, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia; Department of Cardiology and Vascular Medicine, Faculty of Medicine, Airlangga University, Surabaya, 60286, Indonesia; Department of Pharmacology, Rayat Institute of Pharmacy, Distt. S.B.S Nagar, Railmajra, 144533, Punjab, India
