Ni Luh Putu Eka Sudiwati, Tatit Nurseta, Mulyohadi Ali, Aulani’am Aulani’Am, Heru Santoso Wahito Nugroho
DNA methylation is metil group (CH3) adding towards DNA. This mechanism will affected cells function and arise difference gene expression. Tumor suppresor gene hipermethylation, was silencing gene expression, induced cancer progressivity, and interfere the therapy. Recent studies have been reported that some compound from plants could act as an inhibitor on DNA methylation. Since methylation is inhibiting, the silenced tumor suppressor gene caused by hipermethylation, will be re-active. Previous study was succesfully identify flavonoid from Scurrula atropurpurea (Blume) Danser (SAD) that collected from Lawang city, Jawa Timur. Fractionation of a compound have been carried out with a solvent n-heksan, chloroform, and ethanol. Identification of a compound through investigating liquid chromatography mass specthrometry mass (LCMS). The outcome showed that flavonoid compound in SAD were included flavanon, dihidroflavonol, flavon, flavonol, katekin, and Epigallocathecin-3-O-gallate (EGCG). The study was an advanced study, has an objectives to identify the SAD potency towards methylation inhibition, and it was insilico using Autodock Vina on software PyRx 0.8. The result shown that an active compound isolated from SAD have potency to inhibit DNA methylation, in which EGCG as the strongest candidate with binding affinity was-10.4 Kcal./mol. Result of this study can be used to tested a potential active compound of SAD as anticancer both in vitro and in vivo. © 2018, Indian Journal of Public Health Research and Development. All rights reserved.
Department of Nursing, State Health Polytechnic of Malang, Indonesia; Department of Obstetri and Gynecology, Saiful Anwar Hospital, Malang, Indonesia; Faculty of Medicine, Brawijaya University, Malang, Indonesia; Department of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, Indonesia; Laboratory of Organic Chemistry, Mathematic and Natural Sciences, Brawijaya University, Malang, Indonesia; Health Polytechnic of Ministry of Health at Surabaya, Indonesia
