D. Fitriani, M. Cahyati, N. Prasetyaningrum, T.I. Budhy, E. Munadziroh
Tooth extraction can be debilitating due to prolonged inflammation delaying wound healing. Secretory leukocyte protease inhibitor (SLPI) is a non-glycosylated proteomic material that inhibits protease enzymes and has anti-inflammatory properties accelerating wound healing. Increased osteoclast activity is marked by increased osteopontin expression (OPN) during bone resorption. OPN is a phosphorylated glycoprotein secreted by several immune cells. This study aims to determine OPN's expression as an osteoclast activity's overview in wound healing acceleration, following SLPI administration. Thirty-six Rattus norvegicus incisors were extracted and randomly divided into 6 groups by treatment type and duration of observation. SLPI was administered in Group 1 and 2 (12 heads respectively) on the socket of rat's teeth, followed by tight stitches. Group 2 additionally received bone graft. Control group K (12 heads) exclusively received tooth socket stitching. Two observations, each on 6 rats, were performed at 7 and 14 days. Subsequently, biopsies were performed and immunohisto-chemically stained with osteopontin antibodies. One-way Anova and Kruskal-Wallis tests showed statistically significant differences (p<0.05) between the groups. Results suggest that SLPI administration decreases osteoclast activity in alveolar bone resorption by decreasing the number of active osteoclasts and increasing the number of inactive ones in a Rattus model. Osteopontin expression can be used in overview of osteoclast's activity. © 2018 Institute of Physics Publishing. All rights reserved.
Department of Material Science, Faculty of Dentistry, Universitas Brawijaya, East-Java Malang, 65145, Indonesia; Department of Oral Medicine, Faculty of Dentistry, Universitas Brawijaya, East-Java Malang, 65145, Indonesia; Department of Oral Biology, Faculty of Dentistry, Universitas Brawijaya, East-Java Malang, 65145, Indonesia; Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Airlangga, Surabaya, East-Java, 60132, Indonesia; Department of Dental Material, Faculty of Dentistry, Universitas Airlangga, Surabaya, East-Java, 60132, Indonesia
