Yudi Purnomo, Djoko W. Soeatmadji, Sutiman Bambang Sumitro, Moch Aris Widodo
This study was aimed to compare the anti diabetic effect of Urena lobata leaf extract through DPP-4 inhibitory activity by in vitro and in vivo. Urena lobata leaf was extracted in ethanol and hot water to evaluate its activity on DPP-4 both of in vitro and in vivo. In vitro test using Gly-pro-p-nitroanilide (GPPN) as substrate of DPP-4 and the reactions product of them was observed by microplate reader at λ=405 nm furthermore the IC50 value was determined. In vivo study utilize an animal model of diabetes with 2 control groups and 6 test groups (n=4), in which DPP-4 level, GLP-1 level and AUC of blood glucose were examined after extract administration. The in vitro DPP-4 inhibitory activity of ethanolic extract of U. lobata is higher than water extract with the IC50 value of 1654,64 and 6489,88 µg/ml respectively. However, the water extract of U. lobata exhibits stronger decrease DPP-4 level (60-70%) compared to ethanolic extract (40-60%) in vivo study as well as the AUC of blood glucose were reduced by 50-60% and 20-50%, respectively. Meanwhile, GLP-1 level could be retained more by the water extract of U. lobata administration (3-7 fold) compared to ethanolic extract (2-5 fold) due to the reducing of DPP-4 activity. © 2018, IndianJournals.com. All rights reserved.
Department of Pharmacology, Faculty of Medicine, Islamic University of Malang, Jalan MT Haryono No. 193, Malang, 65144, East Java, Indonesia; Department of Internal Medicine, Faculty of Medicine, Brawijaya University, Jalan Veteran, Malang, 65145, East Java, Indonesia; Department of Biology, Faculty of Mathematics and Natural Science, Brawijaya University, Jalan Veteran, Malang, 65145, East Java, Indonesia; Department of Pharmacology, Faculty of Medicine, Brawijaya University, Jalan Veteran, Malang, 65145, East Java, Indonesia
