Nurhayati, Hartutik, Osfar Sjofjan, Eko Widodo
The aims of this research were to evaluate production of mannan oligosaccharides (MOS) extracted from fermented palm kernel cake (PKC) and cassava by-product (CB) mixture using Aspergillus niger and to investigate the efficacy of the extracted MOS in reducing pathogenic bacteria (Salmonella sp. and Escherichia coli) and increasing non-pathogenic bacteria (Lactobacillus sp.) by in vitro test. This research consisted of two trial stages e.i. Trial 1 was to find the best combination between ratio of PKC-CB and length of incubation in producing maximum MOS. Completely Randomised Factorial Design with twenty combinations (4x5) between ratio of PKC-CB (R0 = 100% PKC; R1 = 87.5% PKC: 12.5% CB; R2 = 75% PKC: 25% CB; and R3 = 62.5% PKC: 37.5% CB) and length of incubation (L0 = 0; L1 = 24; L2 = 48; L3 = 72; and L4 = 96 hours) was used in this research. Trial 2 was to evaluate the extracted MOS from the best treatment combination of Trial 1 with dosages of 0 (T0), 1000 (T1), 2000 (T2), 3000 (T3) and 4000 ppm (T4) in reducing pathogenic bacteria and increasing non-pathogenic bacteria by in vitro test. The best dosage of extracted MOS was compared to commercial MOS (T5). Result of this research showed that combination between the ratio of 75% PKC: 25% CB and 72 hours incubation produced the highest MOS. The result also indicated that in vitro test using extracted MOS 4000 ppm was the best in reducing pathogenic bacteria and increasing non-pathogenic bacteria. It can be concluded that MOS extracted from fermented PKC-CB mixture could be an alternative prebiotic. It is suggested that further research should investigate this extracted MOS as poultry feed additive to know its efficacy in improving broiler performance. © 2018, Fundacion CIPAV. All Rights Reserved.
Department of Animal Science, Lampung State Polytechnic, Lampung, Indonesia; University of Brawijaya Malang, East Java, Indonesia; Department of Animal Nutrition and Feed, Faculty of Animal Husbandry, University of Brawijaya Malang, East Java, Indonesia
