Asri Nurul Huda, Hendrawan Soetanto, Akhmad Sabarudin, Anuraga Jayanegara
Rumen biohydrogenation converts dietary unsaturated fatty acids (UFA) into saturated fatty acids (SFA), reducing the nutritional value of ruminant- derived meat and milk. Polyphenol oxidase (PPO) from tropical forages has been proposed as a natural inhibitor of this pathway. In a cross- over in vivo trial with three sheep, we compared: (1) negative control (NC, complete feed); (2) positive control (PC, feed + 1 mL linseed oil); and (3) feed + 3 mL PPO emulsion (from Gliricidia sepium). Carcass fat from the thigh and back was analyzed by gas chromatography. PPO treatment increased linoleic acid (C18:2) in thigh fat by 50% vs NC and 157% vs PC, while stearic acid (C18:0) decreased by 76% vs NC and 22% vs PC. Back- fat linoleic acid rose by 12.5% (NC) and 42.9% (PC), and stearic acid fell by 76.2% and 11.5%, respectively. These findings demonstrate that PPO emulsion effectively protects UFAs from rumen biohydrogenation, yielding a healthier fatty acid profile in sheep meat. The use of plant- derived PPO as a feed additive presents a promising strategy for producing functional ruminant products and mitigating SFA- related health risks. © The Authors, published by EDP Sciences, 2025.
Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia; Faculty of Mathematics and Natural Science, Universitas Brawijaya, Malang, 65145, Indonesia; Department of Nutrition Science and Feed Technology, Faculty of Animal Husbandry, IPB University, Bogor, 16680, Indonesia
