Opportunity plankton as vector transmission of koi herpes virus infection on carp (Cyprinus carpio)

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Uun Yanuhar, Yuliana, Kusriani, Diana Arfiati

2018 AACL Bioflux Vol. 11 Issue 6 Article Cited by 3 Quartile

Abstract

Koi Herpes Virus (KHV) is a disease of carp (Cyprinus carpio) that is influenced by environmental conditions of water. This study aims to determine the vectors in water pond that influence the spread of KHV infection in carp. The method is experimental exploration research with descriptive analysis of water and fish sampling. KHV analysis is done by Polymerase Chain Reaction analysis. Results show the temperature, brightness, pH, dissolved oxygen (DO), CO2, nitrate, orthophosphate, biological oxygen demand (BOD), and total organic matter (TOM) have ranges of 25-27°C, 32-33 cm, 8, 7.11-7.76 mg L-1, 3.58-4.63 mg L-1, 0.688-0.762 mg L-1, 0.046-0.051 mg L-1, 3.986-4.729 mg L-1, and 11.376-13.904 mg L-1, respectively. The compositions of the plankton identified in the pond were a type of phytoplankton, from Chlorophyta division with abundance value and relative abundance of 1405.84 cells L-1 and 63%, respectively, and the zooplankton from Arthropoda phylum had the highest abundance and the relative abundance value of 225.21 cells L-1 and of 71%, respectively. Phytoplankton and zooplankton dominance index are in the range 0.440 to 0.505, and 0.511 to 0.636, respectively. KHV genome on samples of plankton in the water pond and the digestive tract of carp were positively infected with KHV by 292 bp. These results indicate that the planktons are a vector transmission in spreading the KHV in carp in water horizontally. © 2018, BIOFLUX SRL. All rights reserved.

Affiliations

Department of Water Resource Management, Faculty of Fishery and Marine Sciences, Brawijaya University, Jalan Veteran, Malang City, 65145, Jawa Timur, Indonesia