E.D. Kusumawati, S. Rahadi, N. Isnaini, S. Wahjuningsih, A.G. Abdullah, R. Agustina, T. Susilawati
The aim of this study was to determine the quality of sexing sperm before freezing by Percoll density gradient centrifugation method using egg yolks tris aminomethan and andromed® extender. This research method was experimental laboratory studies, the treatment egg yolks tris aminomethan and andromed® extender, parameters observed in the study include motility, percentage of viability and abnormalities fresh sperm before freezing. Sexing results were analyzed using completely randomized design, each treatment consisted of 10 replications. The results showed that both sperm motility and abnormalities using andromed had significant (P <0.05) than using egg yolk tris aminomethan. Glicerol in andromed as a cryoprotectant in the groove mechanism of the reaction cell preservation were to a decrease in freezing cryoprotectant medium, to the protect cell membranes, to curb the influence of increasing concentration, as well as to change the shape and size of ice crystals. It might be concluded that the quality of spermatozoa using andromed better than using egg yolk tris aminomethan. © Published under licence by IOP Publishing Ltd.
Animal Husbandry Study Program, Universitas Kanjuruhan Malang, Jl. S. Supriadi no 48, Malang, 65148, Indonesia; Animal Science Faculty, Universitas Halu Oleo, Kendari, Indonesia; Animal Husbandry Faculty, Brawijaya University, Malang, Indonesia; Departemen Pendidikan Teknik Elektro, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi no 229, Bandung, 40154, Indonesia; Information System Study Program, Universitas Kanjuruhan Malang, Jl. S. Supriadi no 48, Malang, 65148, Indonesia
