Dyah A. O. A. Pratama, Dyah Kinasih Wuragil, Nia Kurniawan
This study was aimed to isolate and characterize the expression of Feline Tetherin/BST-2 protein on PBMCs induced by IFN-α. PBMCs were isolated from blood sample of healthy cats with aged more than 1 year. Samples were divided into two groups, namely the control group and the treatment group which induced by IFN-α at dose of 1000 IU / ml. Total protein was isolated from cultured cells after 24 hours incubations and analyzed using SDS-PAGE, dot blot, and western blotting. The result of SDS-PAGE analysis showed a 35 kDa protein band in treatment group which suggested to be the feline tetherin protein band. The results of dot blot analysis was indicate the positive reaction of antigen Feline Tetherin/ BST-2 and antibody anti BST-2 that marked by purplish blue dot also at treatment group. The western blot analysis showed a confirmation of feline tetherin/BST-2 protein expression with a protein band size approximately 35 kDa molecular weight. Based on the results of this study, can be concluded that administration of IFN-α induction at doses of 1000 IU /ml was able to induce the expression of feline tetherin protein/BST-2 in PBMCs cultured cells, and protein characterization results showed that the feline Tetherin/BST-2 protein has a molecular weight of about 35 kDa. © 2019 Author(s).
Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia; Biology Department, Faculty of Mathematics and Science, Universitas Brawijaya, Malang, Indonesia
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